Journal: iScience

Article Title: PGC-1α in the myofibers regulates the balance between myogenic and adipogenic progenitors affecting muscle regeneration

doi: 10.1016/j.isci.2022.105480

Figure Lengend Snippet:

Article Snippet: Mouse monoclonal anti-Integrin-α7 (clone 3C12), PE-Vio770-conjugated , Miltenyi Biotec , Cat#130-102-718; RRID: AB_2652468.

Techniques: Recombinant, Cell Isolation, cDNA Synthesis, SYBR Green Assay, Isolation, Software

Journal: Pharmacological Research

Article Title: A dual acting compound releasing nitric oxide (NO) and ibuprofen, NCX 320, shows significant therapeutic effects in a mouse model of muscular dystrophy

doi: 10.1016/j.phrs.2011.05.003

Figure Lengend Snippet: NCX 320 increases muscle regeneration, myogenic precursor cells and regenerative potential. (A) Number of centronucleated-regenerating fibres quantified in sections of diaphragm and tibialis anterior muscles; (B) number of CD34/α7 integrin-positive cells isolated from tibialis anterior muscles was measured by flow cytometry; (C) expression of the differentiation markers myogenin (Myog) and myosin heavy chain (MHC) in isolated myogenic precursor cells. All parameters were evaluated in mice treated for 8 months with ibuprofen or NCX 320 incorporated into the diet. Animals receiving the same diet without any drug were used as control. Data are expressed as mean ± SEM ( n = 10). * p < 0.05 vs control animals and # p < 0.05 vs ibuprofen-treated animals. (D) Representative images of the immunoblotting experiments.

Article Snippet: Fluorescein isothiocyanate-conjugated CD34 antibody was purchased from AbD Serotec (Oxford, UK) and phycoerythrin-conjugated α7-integrin antibody from MBL (Woburn, MA, USA).

Techniques: Isolation, Flow Cytometry, Expressing, Western Blot